Josh Lee Holloway first appeared in the Episode: "City Of" of the American television series ‘Angel’ in 1999. He made his big-screen debut in the 2001 erotic thriller film ‘Cold Heart’ directed by Dennis Dimster. The film received mostly mixed reviews. He next appeared in television series and films like ‘Walker, Texas Ranger’ (2001), ‘Sabretooth’ (2002), ‘CSI: Crime Scene Investigation’ (2003), ‘The Lyon's Den’ (2003), ‘NCIS’ (2004), and ‘Good Girls Don't’ (2004). In 2004, he joined the main cast of the American drama television series ‘Lost.’ He played the role of James "Sawyer" Ford in the series which earned him the ‘Saturn Award.’ ‘Lost’ earned praise from the audience and critics alike and came to be known as one of the greatest television series of all time. The show aired its final episode in 2010. In 2011, he starred as ‘IMF Agent Trevor Hanaway’ in the American action spy film ‘Mission: Impossible – Ghost Protocol’ directed by Brad Bird. The film received mostly positive reviews and was a major financial success, grossing over $694.7 million on a budget of $145 million.
In 2013, he appeared in the American 3D dance film ‘Battle of the Year’ directed by Benson Lee. The film was panned by critics and was a box office bomb. The following year, he starred as ‘Gabriel Vaughn’ in the American cyber-themed action-adventure television series ‘Intelligence.’ The series was canceled after one season. In 2016, he became a part of the American science fiction drama television series ‘Colony’ created by Carlton Cuse and Ryan J. Condal. The show ran for 3 seasons before it was canceled in 2018. Holloway was last seen playing the role of ‘Roarke Morris’ in the American drama television series ‘Yellowstone’ in 2020. He is all set to appear in the upcoming television series, ‘Duster.’