Yoo In-na started her acting career in a small role in the South Korean horror thriller film ‘Arang’ in 2006. She went on to have guest roles in the talk shows ‘Happy Together: Season 3’ (2007) and ‘Strong Heart’ (2009) before bagging a breakthrough supporting role as herself in the MBC sitcom ‘High Kick Through the Roof’ (2009-10). In 2010, she became a regular cast member on the variety show ‘Heroes’ and had supporting roles in the romance film ‘The Fair Love’ and the drama series ‘Secret Garden’ (‘Sikeurit Gadeun’), the latter of which earned her a ‘Best New Actress Award’ at the ‘Baeksang Arts Awards’. She gained further recognition after being cast as the second female lead in popular screenwriters Hong sisters' romantic comedy series ‘The Greatest Love’ (‘Choegoui Sarang’) in 2011. That year, she was one of the main casts in the comedy film ‘My Black Mini Dress’, in which she got the opportunity to showcase her singing skills, and had a supporting role in the television series ‘Birdie Buddy’. She also hosted the entertainment information program ‘TV Entertainment Tonight’ as an MC between March 2011 and June 2012, for which she was named the ‘Best Variety Entertainer’ at the ‘SBS Entertainment Awards’.
Yoo In-na landed her first leading role in the 2012 ttime-slip romance series ‘Queen and I’, which earned her the ‘Rising Star Award’ at the ‘APAN Star Awards’, and had a supporting role in the romantic comedy ‘Love Fiction’ (‘Leo-beu-pik-syeon’) that year. In 2013, she was cast as the main female antagonist in the fantasy romantic comedy series ‘My Love from the Star’ (‘Byeol-e-seo on geu-dae’), which helped her gain popularity in China as well. The same year, she had a supporting role in the series ‘You Are the Best!’ (‘Choegoda Lee Soon-shin’) and a cameo appearance on ‘Potato Star 2013QR3’, apart from serving as the narrator for the documentary ‘Little Big Hero’ and an MC for the reality survival series ‘WIN: Who is Next?’
In the following years, Yoo In-na started to get more lead roles in films and on television shows such as the romance-mystery series ‘My Secret Hotel’ (2014), the Chinese romantic comedy movie ‘Wedding Bible’ (2015), and the romantic comedy series ‘One More Happy Ending’ (‘Han-beon deo hae-pi-en-ding’, 2016). She portrayed the attractive chicken restaurant owner Kim Sun a.k.a. Sunny in the fantasy comedy-drama series ‘Guardian: The Lonely and Great God’ (‘Sseulsseulhago Chalranhashin: Dokkaebi’, 2016-17), which was one of the highest rated cable television series in South Korea and earned her a ‘DramaFever Award’ and a ‘Soompi Award’ in the ‘Best Supporting Actress’ category. Furthermore, she had a supporting role in the South Korean-Japanese joint web drama series ‘The Secret Message’ in 2015. During this period, she also had guest roles on television series such as ‘Bubble Gum’ and ‘Top Star U-back’, webseries like ‘We Broke Up’ and ‘YG Future Strategy Office’, and hosted several variety shows including ‘Get It Beauty’ and ‘Cafe Amor’. She was also a regular cast member on ‘Fashion King Korea 3’ and made guest appearance on ‘Candy In My Ear’.
In 2019, Yoo In-na was cast in the lead role of disgraced top actress Oh Jin-shim a.k.a. Oh Yoon-seo in the romance comedy drama ‘Touch Your Heart’ (‘Jinsimi Dadda’), in which she starred alongside her ‘Guardian’ co-star Lee Dong-wook. The same year, she was a regular cast member on the MBC reality show ‘Funding Together’. The following year, she had another lead role as wedding dress designer Kang Ah-reum in the romantic action-comedy series ‘The Spies Who Loved Me’ (‘Naleul Saranghan Seupai’), which earned her several award nominations. That year, she also had a supporting voice role in the comedy-drama film ‘Mr. Zoo: The Missing VIP’ and hosted the variety talk show ‘Love of 7.7 Billion’. She was next cast in the lead role of Lee Hyo-young in the 2021 romantic comedy film ‘New Year Blues’ and played the main role of Kang Chung-ya in the crime drama series ‘Snowdrop’ (‘Seolganghwa’, 2021-22). She has continued to narrate and host various television shows like ‘Still a Brilliant Day’, ‘Together Rewind’, ‘Better Life, Together’, ‘The Museum Is Alive’ and ‘Between Us’, and recently moved to web shows as well, including ‘Charging for Cha Live’ and ‘Hull Watch Party’.